Buy this book on-line

Bazin, H : Rat Hybridomas and Rat Monoclonal Antibodies

CRC Press, Boca Raton, 1990

ISBN 0849354382

8vo - over 7¾" - 9¾" tall. 0849354382 | 515 pgs. PART I: immunocytomas in LOUVAIN (LOU) rats (rat immunoglobulins; the louvain (lou) rats; rat immunocytomas (IR). PART II: production of rat-rat hybridomas (fusion cell lines; immunization of rats; fusion procedure; rat hybridoma production: scanning electron microscopy of a fusion experiment; selective growth of hybrids; cloning; screening of monoclonal antibodies directed against soluble antigens; screening of hybrid supernatants for monoclonal antibodies against cellular antigens; isotyping of rat monoclonal antibodies; adaptation to in vivo culture; freezing and thawing). PART III: production, purification, fragmentation, and labeling of rat monoclonal antibodies (in vitro production of rat monoclonal antibodies; in vivo production of rat monoclonal antibodies; purification of rat monoclonal antibodies from ascitic fluid, serum or culture supernatant; quality control of purified antibodies; fragmenation of rat monoclonal antibodies; labeling of monoclonal antibodies). PART IV: examples of production and characterization of rat-rat hybridomas against various antigens (rat monoclonal antibodies against murine immunoglobulins; allotype specific monoclonal antibodies to rat kappa light chain; application of rat hybridomas to studies on ocular immunobiology; rat monoclonal antibodies against rabbit immunoglobulin; preparation of anti-human igg monoclonal antibodies using rat x rat hybridoma system; rat monoclonal antibodies against human immunoglobulin; production and characterization of rat monoclonal antibodies directed against human apolipoprotein B; studies on rat-rat hybridoma technique and its application to obtain rat monoclonal antibodies anti-horseradish peroxidase; rat-rat and ra-mouse hybridomas secreting rat monoclonal anti-horseradish peroxidase; LOU rat monoclonal antibodies against xenopus vitellogenin; production and characterization of rat-rat hybridomas against DNP-hapten; rat monoclonal antibodies against human leukocyte differentiation antigens; rat monoclonal antibodies specific for t lymphocytes; rat monoclonal antibodies against streptococcus mutans and bacteroides gingivalis; a specific rat monoclonal antibody against yersinia enterocolitica serogroup 0:8; produciton of rat hybridoma directed against yersinia enterocolitica; rat monoclonal antibodies against viral antigens; contribution of rat monoclonal antibodies to the study of schistosoma mansoni infections). PART V: specific uses and properties of rat monoclonal antibodies (mouse immunoglobulin isotyping; humoral immune suppression of mice by monoclonal rat anti-mouse u-heavy chain antibodies; rat monoclonal antibodies against mouse ige; purification of mouse monoclonal antibodies by immunoaffinity chromatography; screening of mouse monoclonal antibodies; use of rat monoclonal antibodies in immunohistochemistry investigation; use of rat-rat monoclonal antibodies labeled with fluorochrome; use of rat monoclonal anti-rat allotype antibodies in immunohistoperoxidase technique and ELISA; use of anti-rat kappa light chain allotype monoclonal antibodies for the quantification of rat monoclonal antibodies in ascitic fluid or serum; extracorporeal removal of arteriogenic particles by use of rat monoclonal antibody coupled to sepharose as specific immunosorbent; antibacteria antigens, especially streptococcus mutans; mercury-induced autoimmunity production of monoclonal antibodies; rat monoclonal antibodies to plant pathogens; rat monoclonal antibodies for complement-mediated and cell-mediated cytolysis; rat antibodies and k cells; absence of viral particles in rat hybridomas. an ultrastructure study; role of codata-hdb in the centralization and distribution of information concerning the rat hybridomas; list of monoclonal antibodies). Usual library markings but otherwise clean and tight. Grey/salmon boards with silver lettering. Clean and tightly bound.. Book. Book Condition: Very Good Ex-Library Cond Book. Binding: Hardcover

Bibliophile Bookbase for antiquarian books, maps and prints.